The Tensile Expansion Microscopy (TExM) cell stretcher: an iris expansion device integrated with automated real-time autofocus and tracking to super-resolve cells

Arampongpun, R.; Shrikanth, T.; Venkataramani, V.; Latham, D. R.; Zammali, M.; Vakil, V.; Kisley, L.e

Submitted

bioRxiv

DOI: 10.64898/2026.09.07.749822

​

Binding and brightness: Spectroscopic evaluation of binding-induced lanthanide sensitization with Lanmodulin-inspired EF peptides

Ambagaspitiya, T. D.; Monge Neria, R.; Biswas, S.; Getman, R.; Renner, J.; Kisley, L.

Submitted, Under Review

​

Tensile Expansion Mass Spectrometry for single cell metabolomics imaging

Guerrero , J.; Older, E. O.; Zammali, M.; Venkataramani, V.; Arampongpun, R.; Latham, D.; Riad, D.; Schwenzfeier, J.; Potthoff, A.; Taylor, D.; Burdette, J.; Andresen Eguiluz, R.; Soltwisch, J.; Kisley, L.*; Sanchez, L.*

Submitted

bioRxiv

DOI: 10.64898/2026.08.15.745024I

Fluorescence detection of Fe(II) senses pitting corrosion differences between 3D-printed and wrought steels

Li, H.; Zhaparov, Z.; Hernandez, K.; Bruckman, L.; Kisley, L.*; Pfaffenberger, Z.*

Submitted, Under Review

ChemRxiv

DOI: 10.26434/chemrxiv.15007237/v1

​

Tensile expansion microscopy applies mechanical force to super-resolve fixed and image live cellular samples

Venkataramani, V.; Latham, D. R.; Arampongpun, R.; Zammali, M.; Shrikanth, T.; Mohapatra, A.; Guerrero, J. A.; Andresen Eguiluz, R. C.; Mathur, D.; Sanchez, L. M.;
Kisley, L.e

bioRxiv

DOI: 10.64898/2026.02.20.707066

​

Polyethylene glycol-induced condensation leads to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor

 Mohapatra, A.; Antarasen, J.; Latham, D. R.; Barilla, M. A.; Davis, C. M.; Kisley, L.

ACS Sensors

2026, 11,  4368–4380.

DOI: 10.1021/acssensors.5c03872

​

A unified analytical method greenness score (uAMGS) quantifies how microscopic imaging is greener than conventional liquid chromatography

Inaba, K.; Monge Neria, R.; Saylor, R. A.; Kisley, L.

ACS Sustainable Chemistry & Engineering

2026, 14, 11058–11067.

DOI:10.1021/acssuschemeng.6c02093

​

The non-linear effects of the number of stochastic single-molecule adsorption events on ensemble elution profiles

 Monge Neria, R.; Saylor, R. A.; Kisley, L.

Analyst

2026, 151, 3974-3986.

DOI: 10.1039/D6AN00199H

​

Visualizing energy transfer between
redox-active colloids

Qu, A. S.; Ou, Z.; Savasatli, Y.; Yao, L.; Cao, Y.; Yu, H.; Montoto, E. C.; Hui, J.; Li, B.; Soares, J. A. N. T.; Kisley, L.; Bailey, B. P.; Murphy, E. A.; Liu, J.; Huang, J.; Evans, C. M.; Schroeder, C. M.; Rodriguez-Lopez, J.; Moore, J. S.; Chen, Q.; Braun, P. V. 

Science Advances

2025, 11, eady7716.

DOI: 10.1126/sciadv.ady7716

​

Electrochemically-assisted low power density laser writing on stainless steel via enrichment of chromium oxide

Pfaffenberger, Z.; Liu, L.; Kim, T. K. J.; Venkataramani, V.; Kisley, L.

Advanced Materials Interfaces

2025, 2500240.
DOI: 10.1002/admi.202500240

​

Super-resolution imaging reveals resistance to mass transfer in functionalized stationary phases

Monge Neria, R.; Zeeshan, M.; Kapoor, A.; Kim, T. K. J.; Hoven, N.; Pigott, J. S.; Gurkan, B.; Duval, C. E.; Saylor, R. A.; Kisley, L.

Science Advances

2025, 11, eads0790.

DOI: 10.1126/sciadv.ads0790

Featured in The Daily, Engineeringness

Effect of polymer gel elasticity on collagen type II fibril assembly

Wilcox, K. G.; Kramer, S.; Chatterjee, S.; Linscott, A.; Suresh, S; Kisley, L.; Morozova, S.

Soft Matter

2025, 21, 137-147.

DOI: 10.1039/D4SM01104J

Quantitative advantages of corrosion sensing using fluorescence, microscopy, and single-molecule detection

Siegel, M.; Liu, L.; Pfaffenberg, Z.; Kisley L.

ACS Applied Materials and Interfaces

2024, 16, 56481–56496.

DOI: 10.1021/acsami.4c07800

D

A practical guide to light-sheet microscopy for nanoscale imaging:
Looking beyond the cell

Kramer, S. N.; Antarasen, J.; Reinholt, C.; Kisley L.

Journal of Applied Physics

2024, 136, 091101.

DOI: 10.1063/5.0218262

Cross-correlation increases sampling in diffusion-based super-resolution optical fluctuation imaging

Antarasen, J.*; Wellnitz, B.*; Kramer, S. N.; Chatterjee, S.; Kisley L.

Chemical and Biomedical Imaging

2024, 2, 640-650.

DOI: 10.1021/cbmi.4c00032

*Equal Contribution​

A turn-off fluorescent sensor for metal ions quantifies corrosion in an organic solvent

Liu, L; Pfaffenberger, Z; Seigal, M; Saini, A; Kisley,  L.

Journal of the Electrochemical Society

2024, 171, 051502.
DOI: 10.1149/1945-7111/ad441f

​

Spatially resolving size effects on diffusivity in nanoporous extracellular matrix-like materials with fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI)

Chatterjee, S.*; Kramer, S.*; Wellnitz, B.; Kim, A.; Kisley, L.
The Journal of Physical Chemistry B
2023, 127, 4430-4440.

DOI: 10.1021/acs.jpcb.3c00941

*Equal Contribution

Invited Article, Young Investigator Issue

Peroxisome biogenesis initiated by protein phase separation

Ravindran, R.; Bacellar, I. O. L.; Castellanos-Girouard, X.; Wahba, H. M.; Zhang, Z.; Omichinski, J. G.; Kisley, L.; Michnick, S. W.
Nature

2023, 617, 608-615.
DOI: 10.1038/s41586-023-06044-1

Reduction reactions at metal/non-aqueous interfaces can be sensed with the turn-on fluorophore resazurin

Gatland, Z.; Madrid, D.; Siegel, M.; Kisley, L.

Materials Chemistry Frontiers

2023, 7, 2260-2265.

DOI: 10.1039/D2QM01309F

Invited Article - Frontiers Emerging Investigator Series

Native diffusion of fluorogenic turn-on dyes accurately report interfacial chemical reaction locations

Messenger, H.; Madrid, D.; Saini, A.; Kisley, L.

Analytical and Bioanalytical Chemistry

2023, 415, 4479–4486.

DOI: 0.1007/s00216-023-04639-1

Invited Article - Young Investigators in (Bio-)Analytical Chemistry 2023 Issue

Single-molecule imaging in commercial stationary phase particles using highly inclined and laminated optical sheet microscopy

Monge Neria, R.; Kisley, L.

Analytical Chemistry

2023, 95, 2245–2252.

DOI: 10.1021/acs.analchem.2c03753

Towards protein chromatography by design: Stochastic theory, single-molecule parameter control, and stimuli-responsive materials

Dutta, C.; Misiura, A.; Bishop, L. D. C.; Marciel, A. B.; Kisley, L.; Landes, C. F.

The Journal of Physical Chemistry C
2022, 126, 18587–18595.

DOI: 10.1021/acs.jpcc.2c05887

A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories

Götz,M.; Barth, A.; Bohr, S. S.-R.; Börner, R.; Chen, J.; Cordes, T.; Erie, D.A.; Gebhardt, C.; Hadzic, M. C.A.S.;   Hamilton, G.; Hatzakis, N. S.; Hugel, T.; Kisley, L.; Lamb, D. C.; de Lannoy, C.; Mahn, C.; Mudiyanselage, D. D.; de Ridder, D.; Sanabria, H.; Schimpf, J.; Sigel, R. K. O.; Sletfjerding, M. B.; Thomsen, J.; Vollmar, L.; Wanninger, S.; Weninger, K. R.; Xu, P.; Schmid, S.

Nature Communications

2022, 13, 5402.

DOI: 10.1038/s41467-022-33023-3

Investigation of fluorophores for single-molecule detection of anodic corrosion redox reactions.

Saini, A.; Gatland, Z.; Begley, J.; Kisley, L.

MRS Communications

2021, 11, 804-810.

DOI: 10.1557/s43579-021-00096-y

Remote exploration of experimental biophysical instrumentation in core facilities.

Kisley, L. 

The Biophysicist

2021, 2, 2-10.

DOI: 10.35459/tbp.2021.000189

Super-resolution fluorescence imaging of extracellular environments

Yoshida, S.; Kisley, L.

​Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

2021, 257, 119767.

DOI: 10.1016/j.saa.2021.119767

Invited Article

Computationally-efficient spatiotemporal correlation analysis super-resolves anomalous diffusion

Yoshida, S.*; Schmid, W.*; Vo, N.; Calabrase, W.; Kisley, L.

Optics Express

2021, 29, 7616-7629.

DOI: 10.1364/OE.416465

* - Equal Contribution

Fluorophores “turned-on” by corrosion reactions can be detected at the single-molecule level

Saini, A.; Messenger, H.; Kisley, L.

​ACS Applied Materials and Interfaces

2021, 13, 2000–2006.

DOI: 10.1021/acsami.0c18994

Transforming separation science with single-molecule methods

Calabrase, W.; Bishop, L. D. C.; Dutta, C.; Misiura, A.; Landes, C. F.; Kisley, L.

Analytical Chemistry

2020, 92, 13622-13629.

DOI: 10.1021/acs.analchem.0c02572

One of the “Most Read Articles” in 2020, Selected as ACS Editor’s Choice, LiveSlides summary

Real-time measurement of polymer brush dynamics using silicon photonic microring resonators: Analyte partitioning and interior brush kinetics

Wetzler, S. P.; Miller, K. A.; Kisley, L.; Stanton, A. S.; Braun, P. V.; Bailey, R. C.

Langmuir

2020, 36, 10351-10360.

DOI: 10.1021/acs.langmuir.0c01336

Fluorescence microscopy of biophysical protein dynamics in nanoporous hydrogels

Saini, A.; Kisley, L.

Journal of Applied Physics

2019, 126, 081101.

Invited Article, Featured News Story: AIP Publishing Scilight

DOI: 10.1063/1.5110299

Single molecule spectroscopy at interfaces.

Kisley, L.

In Spectroscopy and Dynamics of Single Molecules: Methods and Applications.

Johnson, C. K., Ed.; Elsevier: Amsterdam, 2019; pp 117-161.

Unifying chromatography on all fronts

Kisley, L.

Unifying chromatography on all fronts

The Analytical Scientist

2019, 76, 16-17.

Probing inhomogeneous diffusion in the microenvironments of phase-separated polymers under confinement

Shayegan, M.; Tahvildari, R.; Metera, K.; Kisley, L.; Michnick, S. W.; Leslie, S. R.

Journal of the American Chemical Society

2019, 141, 7751-7757.

DOI: 10.1021/jacs.8b13349