1/18/2023 - Excited to share Ricardo’s new work in Analytical Chemistry – using an optical design originally used in cells, we performed 3D super-resolution imaging of single analyte dynamics within chromatography stationary phase materials. This allows us to image commercial – not model – materials used in columns at the single-molecule scale to better connect fundamental observations to applications. Check it out here: https://doi.org/10.1021/acs.analchem.2c03753 Congratulations!
Lab awarded Allen Distinguished Investigator funding
12/15/2022 - The Paul G. Allen Frontiers Institute has named Lydia an Allen Distinguished Investigator along with our collaborator Prof. Laura Sanchez at UC Santa Cruz. We look forward to developing new ways to sense nutrients in cells using expansion with the support. Learn more here from the Allen Institute and from our feature in the Case Daily.
11/11-12/8/2022 - Lydia has a number of upcoming presentations if you are interested in the latest and greatest work going on in the lab. Several will have Zoom options if you want to join remotely. Please go to the host department website for information.
Friday, 11/11, U. Arkansas Analytical Seminar, via Zoom, 5 pm EST
Tuesday, 11/15, U. Akron, Chemical, Biomolecular and Corrosion Engineering, 1 pm EST
Thursday, 11/17, CWRU Physics Colloquium, Rock 301, 4 pm EST
Wednesday, 11/30, Rice U., Chemistry Kinsey Lecture, 5 pm EST
Thursday, 12/8, CWRU Biology Colloquium, DeGrace Hall, 4 pm EST
Stories of Science in the Cleveland Cultural Gardens
10/29/2022 - Lydia and Tiffany shared the results of Tiffany's summer research on "Jeanette Grasselli Brown and Using Light to Understand the Mystery of Molecules" with the Hungarian Garden. We were lucky enough to have Jenny join us for the event too!
Congrats Zack on your next endeavor!
10/14/2022 - We had a send off for Zack as he leaves the lab for a job in industry at Mactac. Congrats on the new position and you will be missed in the lab!
Lydia attends SciX
Lydia is presenting the lab's work on separations and corrosion at the annual Federation of Analytical Chemistry and Spectroscopy Societies' SciX conference in Covington, KY. She's especially looking forward to sharing Ricardo's work during the FACSS Innovation Award competition that recognizes " the most innovative and outstanding new research advancements debuted orally at the SciX Conference." If you're in Kentucky we hope to see you there!
CWRU Innovation Award
Lydia and the hard work of the lab have been recognized in CWRU's inaugural Innovation Week with a "Faculty Innovator Award." We will be recognized at a campus event on September 15. You can learn more about Innovation Week from CWRU here.
Fall ACS National Meeting
Lydia is hosting the PHYS symposium "Standing Out in the Crowd: Dynamics of Biomolecules in Complex Environments" along with Caitlin Davis and Julie Biteen M-W and giving talks on corrosion (W) and chromatography (R). Hope to see you in Chicago!
Congratulations go to Lydia on being recognized by 3M's Non-Tenured Faculty Award (NTFA)!
The NTFA from 3M recognizes excellent junior faculty members who have been nominated by 3M researchers for their demonstrated record of research, experience, and academic leadership. The award will help support our lab’s research activities in imaging porous materials. Lydia will present her research to 3M's corporate headquarters later this year along with the other national awardees.
Lab Lunch Outing
We had a fun time out at Goldhorn Brewery with the Saini group to celebrate the mid-summer point of research!
We're happy to have our collaborator Sarah Levy join us from UCSC for the month to help establish a cell line for our Scialog collaboration with Prof. Laura Sanchez' group. We look forward to working with you!
Surajit's 1st place poster
Congrats to Dr. Surajit Chatterjee on being awarded 1st place in the poster session at the Cleveland Center for Membrane & Structural Biology Symposium! Surajit shared his past research on DNA dynamics w/ Prof. Nils Walter's lab & his current work imaging biomolecule dynamics in the ECM. Way to represent the lab & also show that nanoscale dynamics are just as informative as static, Angstrom-level images :)
Congrats to lab alum, Will Schmid, on his NSF GRFP
We’re happy to celebrate the successes of both present and former group members. It was great to hear Will Schmid (CWRU Physics, Class of 2020) was awarded an NSF Graduate Research Fellowship! Will worked in the Kisley Lab from 2019-2020 on improving the accessibility and functionality of the fcsSOFI analysis (See his publication: Computationally-efficient spatiotemporal correlation analysis super-resolves anomalous diffusion.) The fellowship will support his current PhD work in the department of Electrical and Computer Engineering at Rice University with Prof. Alessandro Alabastri.
Congratulations - Zack passes his Masters thesis defense!
Lydia shares scientific outreach with Cleveland-Bratislava Sister Cities organization
Lydia spoke about the scientific pursuits of Gen. Milan Štefánik, Cleveland’s Cultural Garden connection & how global & local history inspires interest in STEM at the Cleveland-Bratislava Sister Cities organization (hosted at the Cuyahoga Public Library Independence Branch).
Lydia attends ACS in San Diego
Group attends APS in Chicago
Dr. Stephanie Kramer has joined the lab as a postdoctoral researcher after recently completing her PhD at Carnegie Mellon. Welcome to the lab!
Happy International Day of Women & Girls in Science
The lab’s research was featured on WKYC Channel 3 News and Lydia shared some of what inspired her to go into science for CWRU’s College of Arts and Sciences.
Lydia presents virtually at Pacifichem
We’re happy to have Dr. Surajit Chatterjee join our group as a postdoctoral researcher. Welcome!
Awarded “Scialog: Chemical Machinery of the Cell” funding
Thanks to the Research Corporation for Science Advancement and the Gordon and Betty Moore Foundation for awarding our group funding. The award will support an interdisciplinary project with Dr. Laura Sanchez and UC Santa Cruz and Dr. Ronit Freeman at UNC Chapel Hill to apply our expansion microscopy/hydrogel stretcher device to “Stretching Reality to Discover the (un)Knowns.” We’re looking forward to the collaboration and getting to work! You can find out more about the RCSA Scialog awards here: https://rescorp.org/news/2021/12/scialog-chemical-machinery-of-the-cell-final-round-of-awards-tops-1.3m
Preprint on FRET analysis is now live!
Happy to be part of the kinSoftChallenge - a blind study of single-molecule fluorescence resonance energy transfer (smFRET) kinetic analysis algorithms. Observing the movements of one single molecule over time provides information of the underlying energetic origin of protein function at the (sub-)molecular level. Yet, experimental & fundamental limitations complicate the extraction of this important information. The challenge compared different kinetic softwares to determine what the strengths and weaknesses of each were for analyzing data complicated by noise, multiple states, or degeneracies. The results of this multi-team study are now available via BioRxiv and are under peer review. Check out the results here! https://doi.org/10.1101/2021.11.23.469671
Presentation at University of Akron, Department of Chemistry
Lydia participates in Scialog: Chemical Machinery of the Cell virtual workshop
Lydia presents at 2nd Wintergreen Meeting of Physical Chemists
It’s now a biennial tradition! Lydia headed to western Virginia to share the group’s recent progress. She had a lot of fun seeing old colleagues and meeting new postdocs in the field. A lot of exciting science was discussed - attending meetings in-person is awesome!
Research on imaging the extracellular matrix funded by NIH NIGMS!
Big news for the lab, as we are now NIH funded! We have received an R35 Maximizing Investigators’ Research Award for Early Stage Investigators (MIRA ESI) from the NIH - National Institute of General Medical Sciences. The funding will support our research over the next five years imaging protein dynamics in the extracellular matrix. We’re looking forward to the discoveries to be made!
Good luck to Zhenghao!
We bid adieu to master student Zhenghao as he heads to Texas A&M for a PhD in physics. Congratulations!
Shawn, Hannah, and Lydia featured in art/sci magazine
Mentorship and undergraduate research in the lab during the pandemic was featured in the CWRU College of Arts and Sciences Spring/Summer 2021 magazine. You can check it out here: https://artsci.case.edu/magazine/issue/spring-summer-2021/
Pedagogy paper in The Biophysicist published!
Teaching during COVID was an adventure, but led to some creative assignments. Lydia has a new publication featuring how core facilities can be used in instrumentation courses remotely. The work is in The Biophysicist, a journal published by the Biophysical Society. Thanks to all the PHYS 330/430 students from Fall 2020 for their comments on this assignment. Check out the manuscript here: https://doi.org/10.35459/tbp.2021.000189
Shawn and Hannah receive departmental awards
We are very proud of our recent group alumni Shawn Yoshida and Hannah Messenger on their physics department awards which recognize their excellence in research and service to the department. Shawn received the Wyant award for resarch accomplishments outside of senior project, while Hannah received the Foldy Award for an outstanding senion in physics and the Garwin award for service and scholarship in physics. Congratulations!
Lydia hosts Colloids and Interfaces in Biology and Medicine sessions at the virtual 95th ACS Colloid and Surface Science Symposium
More details about the conference can be found at https://sites.psu.edu/2021colloids/
Congratulations Bill on your next endeavors
Congratulations to group member Bill Calabrase on completing his time at CWRU with a Masters degree in Physics. While we are sad to see you go, we know you’ll be off to do great things!
Group attends Probing Chemical Reactions by Single-Molecule Spectroscopy 2021 conference
With a great line-up of speakers, the group attended the single-molecule conference virtually. Lydia also presented on the group’s work on imaging and modeling corrosion at the single molecule scale. We learned a lot and can’t wait for the next conference (with the hope it will be in person)! Thank you to Randy and Suzanne for organizing an engaging meeting.
Anuj receives Microscopy and Microanalysis award
Congrats to group postdoctoral researcher Dr. Anuj Saini who received a Microscopy and Microanalysis (M&M) Postdoctoral Award! Microscope Looking forward to your presentation at the August M&M conference: https://microscopy.org/MandM/2021/
Lydia presents at Life, the Universe, and Hot Dogs
Lydia talked about the scientific life of Milan Štefánik and the history of his statue in the Slovak cultural garden in Cleveland. Video link of the talk can be found here: https://youtu.be/kW8-f5G4atI
Congrats to the grads, Shawn and Andrew!
It was a celebratory but also somewhat sad day in lab, as we sent off seniors Shawn (Physics, 2021) and Andrew (Engineering Physics, 2021). They accomplished a lot during their time in the group. Shawn was the OG member since 2019 and worked on the fcsSOFI project, publishing our first analysis paper in Optics Express and code available on GitHub. He mastered the art of presenting on Zoom throughout the past year. Andrew was a jack of all trades on a project that required engineering and interdisciplinary work. He used ThinkBox laser cutters and 3D printers to build a gel stretcher and learned some polymer chemistry with hydrogel synthesis. He took a project that was an idea on paper and made it a reality! We wish them the best as they pursue their PhDs at Caltech & UIUC, respectively!
Andrew, Anoushka, and Shawn present at Intersections
Congratulations to the undergraduate researchers in our group on their successful year of research. To learn more about what they accomplished, please attend CWRU’s Virtual Intersections and Celebration of Student Writing and Research.
You can find out more and access the live and recorded talks here: https://case.edu/source/share-your-work/welcome-virtual-intersections-and-celebration-student-writing-and-research
and on YouTube:
Group awarded funding though 2021 Expanding Horizons Initiative!
In collaboration with Prof. Christine Duval’s group in Chemical and Biomolecular Engineering, we are happy to share our Interdisciplinary Grant project “Imaging and Designing Better Separations for Radiochemical Elements” has been funded through CWRU’s Expanding Horizons Initiative. You can find out more here: https://artsci.case.edu/expanding-horizons-initiative/. We are looking forward to working on this project through the next year and thank the College of Arts and Sciences and Dean Ward for support.
Anuj presents at MRS
Anuj will be presenting about our work on single molecule corrosion detection at the Materials Research Society spring meeting on Thursday 4/22, at 10:25 pm. You can find the details here:
Lydia and Shawn’s perspective on super-resolution imaging in extracellular environments has been accepted in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy! You can access the “Just Accepted” version here: https://doi.org/10.1016/j.saa.2021.119767
Anuj and Shawn present at APS March Meeting
If you’re at the virtual meeting, we hope you’ll stop by and hear about the new research from our group. Shawn is presenting on Thursday in V12: Instrumentation and Techniques and Anuj on Friday in Session X10: Chemical Physics on the Nanoscale
Anoushka awarded SOURCE WiSTEM funding!
Congratulations to undergraduate group member Anoushka for her proposal “Incorporating Diffusion into Modeling of Separations” being supported through the SOURCE Semester Award program. This is generously supported through the Case Alumni Association.
Optics Express publication on GPU-parallelized fcsSOFI
Congrats to Shawn, Will, Nam, and Bill on their publication and software package! fcsSOFI is our correlation-based analysis code that allows for super-resolution imaging of diffusion dynamics. We’ve improved the computation time by 40x and expanded the diffusion models to image anomalous diffusion in biophysically-relevant environments. We hope that the user-friendly GUI will allow other researchers to apply our analysis to their own research.
Lydia nominated for the J. Bruce Jackson, M.D., Award for Excellence in Undergraduate Mentoring!
Happy Holidays - our first research article on corrosion detection is now live!
Find it here: https://pubs.acs.org/doi/10.1021/acsami.0c18994
Congrats to Anuj and Hannah! We show that corrosion can be monitored with dyes that can be detected at the single molecule scale. We first characterized the dye turn-on of Fe corrosion in bulk, resolving color & fluorescence changes, along with quantifying kinetics. Then we moved onto the microscopic detection showing we can observe single molecule corrosion events with our fluorophore readout on our TIRF microscope. Previous fluorescence corrosion detection was therefore only limited by the optical components used.
This work is the first step in what we hope is a new way to understand corrosion at the ultimate limit of detection. We have more work in the pipeline on the diffusion of our dye & are looking forward to studying the spatial & kinetic dynamics of corrosion at the single-molecule level.
Kisley Group sweeps the Intersections conference physical science awards!
Big congrats to group members Hannah and Shawn for having their talks recognized with the Physical Science presentation awards at the December Intersections: SOURCE Symposium! Way to represent the group!
Congrats to Hannah!
Congratulations were in order at our last group meeting of the semester for Hannah’s graduation from CWRU. Hannah achieved a lot during her 2020 senior project in our group. She shifted from experiment to modeling corrosion and shared her results via a poster and presentation at CWRU Intersections. She is also a co-author on a manuscript from our lab. We wish you the best of luck in your future endeavors!
Shawn’s talk selected as Honorable Mention at GCURS!
Congrats to Shawn Yoshida for winning an honorable mention award for his presentation at the 2020 Gulf Coast Undergraduate Research Symposium. The session was held virtually on Saturday, October 31 and Shawn shared his research on imaging protein diffusion in confined environments.
Lab participates in Sciencepalooza!
We enjoyed the outreach opportunity through the CWRU Science and Human Rights Coalition graduate student organization. We shared our labs microscopy and simulation methods with students from John Hay High School.
ACS Petroleum Research Fund grant funded!
We’re excited to share that our grant “Single Molecule Imaging of Catalysis in Complex Petroleum Environments” has received funding from the American Chemical Society. We’re looking forward to studying turn-on fluorescence in new solvents and mixtures starting in 2021!
Shawn presents at Ohio APS meeting
Lydia featured on Channel 19 Women in STEM interview
Thanks to Kelly Dobeck for featuring our research on Cleveland’s Channel 19. Check out the video here: https://www.cleveland19.com/video/2020/10/05/first-alert-weather-school-women-stem-researching-northeast-ohio/
Lydia and Shawn attend Gibbs34
10/4/2020 - 10/5/2020
No trip to Southern Illinois this year, but we still made it the conference virtually! Lydia co-chaired the poster sessions of the 34th Gibbs Conference on Biological Thermodynamics, while Shawn presented his work “Characterizing the simulated anomalous diffusion of proteins in relation to the nanoporous structure of extracellular matrix-relevant hydrogels.”
Perspective published in Analytical Chemistry
Congrats to Bill on his first publication “Transforming separation science with single-molecule methods.” Written in collaboration with the Landes group at Rice, we present our perspective on future ways single molecule microscopy can contribute to our understanding of separations. We discuss ways that instrumentation improvements (multicolor, 3D imaging, time resolution), sample complexity (mixtures, high concentrations, solvents), & expanding the types of separations studied at the single molecule level (chiral, radiochem, etc.) can be applied in new ways.
Publication in Langmuir
Check out our collaborative paper with Ryan Bailey’s group at Michigan and Paul Braun’s group at Illinois showing how different polymer brush-analyte properties can be quantified with a single tool: the microring resonator. pKa, partition coefficients, diffusion constants, brush thickness, and analyte penetration depth can all be determined.
Presentation at ACS Fall Virtual Meeting
Lydia will be presenting Tuesday morning at 11:10 Eastern/8:10 Pacific in the “Single-molecule microscopy of molecular search processes: from fundamental biophysics inquiry, to pharmaceutical applications” session. Zoom is available for anyone to attend!
Will’s last day & send off
While we are sad to see him go, we wish group member Will Schmid the best of luck in his graduate studies at Rice University! Thanks for all of your contributions to the group!
Diversity discussion & statement
Our group has been reflecting on diversity and inclusion in science and the broader world. We have had multiple discussions on how diversity and representation relates to our role as a research group and as individual scientists. We have incorporated our reflection in a diversity statement now available on our website. We also include resources and opportunities that we encourage our members to participate in to make science and physics a more equitable and better place!
Lydia selected as a Scialog Fellow
Lydia is excited to learn she will be able to participate in the 2020/2021 Scialog: Chemical Machinery of the Cell initiative sponsored by the Research Corporation for Science Advancement (RCSA) and the Gordon and Betty Moore Foundation. Scialog meetings support early career faculty, encourage scientists to form multidisciplinary teams, and provide award opportunities and support to obtain further funding for innovative ideas. She’s looking forward to the first meeting in October!
Rising 2nd year PhD physics student Ricardo has joined the group to study 3D single molecule imaging within porous materials. We’re looking forward to working with you!
Lydia receives the Spectroscopy Society of Pittsburgh’s Starter Grant Award
Lydia is thankful to have received an instrumentation grant in support of our research efforts. The Starter Grant Award from the Spectroscopy Society of Pittsburgh is given annually to an assistant professor who promotes the training and development of graduate students in the fields of spectroscopy. The grant will be used to expand our instrumental capabilites to three-dimensional, multicolored imaging of single molecules.